Elucidation of muscle‐binding peptides by phage display screening
Identifieur interne : 001195 ( Istex/Checkpoint ); précédent : 001194; suivant : 001196Elucidation of muscle‐binding peptides by phage display screening
Auteurs : Tatiana I. Samoylova [États-Unis] ; Bruce F. Smith [États-Unis]Source :
- Muscle & Nerve [ 0148-639X ] ; 1999-04.
English descriptors
- Teeft :
- Adenoviral vectors, April, Asslnia, Asslnia peptide, Asslnia phage, Blood cells, Cardiac, Cardiac muscle, Cell binding, Clone, Control organs, Control phage, Control tissue, Direct gene transfer, Gene therapy, Intravenous injection, Ligand, Murine, Muscle cells, Muscle nerve april, Myotubes, Peptide, Phage, Phage clone, Phage display, Phage display library, Skeletal muscle, Vivo, Vivo screening.
Abstract
Muscle makes up the largest tissue volume of the body, yet its size makes muscle‐specific therapy difficult. This becomes particularly relevant when approaches to gene therapy for inherited myopathies are evaluated. Thus, a mechanism to target constructs or pharmaceuticals to muscle following intravenous injection would be advantageous. By screening a random phage display library we have identified a heptapeptide sequence, ASSLNIA, with enhanced in vivo skeletal and cardiac muscle binding. Phage carrying this peptide showed a 9‐ to 20‐fold (depending on control tissue) increase in muscle selectivity compared with phage with no insert. When the injected individual phage clone was localized by immunohistochemistry, it was found within focal areas of the membrane of myofibers. Thus, the peptide identified represents a ligand that is capable of accessing skeletal and cardiac muscle from the lumen of blood vessels and could therefore readily be exploited for targeted delivery to muscle cells. © 1999 John Wiley & Sons, Inc. Muscle Nerve 22: 460–466, 1999.
Url:
DOI: 10.1002/(SICI)1097-4598(199904)22:4<460::AID-MUS6>3.0.CO;2-L
Affiliations:
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ISTEX:8E8844193E37647BCA898F3B62401CD1118408E0Le document en format XML
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Smith</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8E8844193E37647BCA898F3B62401CD1118408E0</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1002/(SICI)1097-4598(199904)22:4<460::AID-MUS6>3.0.CO;2-L</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-RL7R6G2Z-S/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000E98</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000E98</idno><idno type="wicri:Area/Istex/Curation">000E98</idno><idno type="wicri:Area/Istex/Checkpoint">001195</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001195</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Elucidation of muscle‐binding peptides by phage display screening</title><author><name sortKey="Samoylova, Tatiana I" sort="Samoylova, Tatiana I" uniqKey="Samoylova T" first="Tatiana I." last="Samoylova">Tatiana I. Samoylova</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Scott‐Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849</wicri:regionArea><wicri:noRegion>Alabama 36849</wicri:noRegion></affiliation></author><author><name sortKey="Smith, Bruce F" sort="Smith, Bruce F" uniqKey="Smith B" first="Bruce F." last="Smith">Bruce F. Smith</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Scott‐Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849</wicri:regionArea><wicri:noRegion>Alabama 36849</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Correspondence address: Scott‐Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849</wicri:regionArea><wicri:noRegion>Alabama 36849</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Muscle & Nerve</title><title level="j" type="alt">MUSCLE AND NERVE</title><idno type="ISSN">0148-639X</idno><idno type="eISSN">1097-4598</idno><imprint><biblScope unit="vol">22</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="460">460</biblScope><biblScope unit="page" to="466">466</biblScope><biblScope unit="page-count">7</biblScope><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1999-04">1999-04</date></imprint><idno type="ISSN">0148-639X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0148-639X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adenoviral vectors</term><term>April</term><term>Asslnia</term><term>Asslnia peptide</term><term>Asslnia phage</term><term>Blood cells</term><term>Cardiac</term><term>Cardiac muscle</term><term>Cell binding</term><term>Clone</term><term>Control organs</term><term>Control phage</term><term>Control tissue</term><term>Direct gene transfer</term><term>Gene therapy</term><term>Intravenous injection</term><term>Ligand</term><term>Murine</term><term>Muscle cells</term><term>Muscle nerve april</term><term>Myotubes</term><term>Peptide</term><term>Phage</term><term>Phage clone</term><term>Phage display</term><term>Phage display library</term><term>Skeletal muscle</term><term>Vivo</term><term>Vivo screening</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Muscle makes up the largest tissue volume of the body, yet its size makes muscle‐specific therapy difficult. This becomes particularly relevant when approaches to gene therapy for inherited myopathies are evaluated. Thus, a mechanism to target constructs or pharmaceuticals to muscle following intravenous injection would be advantageous. By screening a random phage display library we have identified a heptapeptide sequence, ASSLNIA, with enhanced in vivo skeletal and cardiac muscle binding. Phage carrying this peptide showed a 9‐ to 20‐fold (depending on control tissue) increase in muscle selectivity compared with phage with no insert. When the injected individual phage clone was localized by immunohistochemistry, it was found within focal areas of the membrane of myofibers. Thus, the peptide identified represents a ligand that is capable of accessing skeletal and cardiac muscle from the lumen of blood vessels and could therefore readily be exploited for targeted delivery to muscle cells. © 1999 John Wiley & Sons, Inc. Muscle Nerve 22: 460–466, 1999.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Samoylova, Tatiana I" sort="Samoylova, Tatiana I" uniqKey="Samoylova T" first="Tatiana I." last="Samoylova">Tatiana I. Samoylova</name></noRegion><name sortKey="Smith, Bruce F" sort="Smith, Bruce F" uniqKey="Smith B" first="Bruce F." last="Smith">Bruce F. Smith</name><name sortKey="Smith, Bruce F" sort="Smith, Bruce F" uniqKey="Smith B" first="Bruce F." last="Smith">Bruce F. Smith</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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